Fluorescence microtomography: multiangle image acquisition and 3D digital reconstruction

Carol J. Cogswell; Kieran G. Larkin; Hanno U. Klemm

doi:10.1117/12.237467

10 April 1996 Fluorescence microtomography: multiangle image acquisition and 3D digital reconstruction

Carol J. Cogswell, Kieran G. Larkin, Hanno U. Klemm

Proceedings Volume 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III; (1996) https://doi.org/10.1117/12.237467
Event: Electronic Imaging: Science and Technology, 1996, San Jose, CA, United States

Abstract

We have developed a prototype fluorescence microscope which, using tomographic image acquisition and reconstruction techniques, can automatically combine conventional and/or confocal image stacks taken at a number of orientations into a single, very-high-resolution 3D image. We use the term `microtomography' in a broad sense to denote digital image reconstruction from multiple imaging operations which are not necessarily projections. Our system holds a biological specimen inside a thin capillary tube which is rotatable over a 360 degree range beneath an immersion objective. 3D fluorescence image data volumes are acquired by frame-grabbing a through-focus series of 2D images at each angle of rotation. Digital reconstruction of the multi-angle data volumes produces a single very-high-resolution 3D image and involves algorithms which perform rotation, interpolation, alignment and normalization operations in frequency (Fourier) space.

Citation Download Citation

Carol J. Cogswell, Kieran G. Larkin, and Hanno U. Klemm "Fluorescence microtomography: multiangle image acquisition and 3D digital reconstruction", Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, (10 April 1996); https://doi.org/10.1117/12.237467

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available