Internal absorption coefficient and threshold for pulsed laser disruption of melanosomes isolated from retinal pigment epithelium

Steven L. Jacques; Randolph D. Glickman; Jon A. Schwartz

doi:10.1117/12.239608

7 May 1996 Internal absorption coefficient and threshold for pulsed laser disruption of melanosomes isolated from retinal pigment epithelium

Steven L. Jacques, Randolph D. Glickman, Jon A. Schwartz

Author Affiliations +

Proceedings Volume 2681, Laser-Tissue Interaction VII; (1996) https://doi.org/10.1117/12.239608
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

This study determine the threshold radiant exposure from a 10-ns pulsed laser (532 nm) which caused bovine melanosomes to present various observable endpoints of disruption. The endpoints tested were (1) a visible region of clearing in a uniform field of melanosomes under a glass cover slip, (2) an audible sound, and (3) the increase in melanin photoreactivity due to photodisruption of melanosomes. The thresholds were tested for different starting temperatures by pre-equilibrating the melanosomes in aqueous solution at various temperatures. Lower temperatures required larger exposures to attain a given endpoint. The data suggest that bovine RPE melanosomes are about 4-fold denser in melanin content than cutaneous melanosomes.

Citation Download Citation

Steven L. Jacques, Randolph D. Glickman, and Jon A. Schwartz "Internal absorption coefficient and threshold for pulsed laser disruption of melanosomes isolated from retinal pigment epithelium", Proc. SPIE 2681, Laser-Tissue Interaction VII, (7 May 1996); https://doi.org/10.1117/12.239608

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