Increase in reactive oxygen species production and phagocytic activity of polymorphonuclear neutrophils stimulated by preirradiated hematoporphyrin-derivative solution

Vladislava Melnikova; Lina N. Bezdetnaya; Irina Belitchenko; Alla A. Kyagova; Pascal Colosetti; Alexander Ya. Potapenko; Francois H. Guillemin

doi:10.1117/12.273496

8 May 1997 Increase in reactive oxygen species production and phagocytic activity of polymorphonuclear neutrophils stimulated by preirradiated hematoporphyrin-derivative solution

Vladislava Melnikova, Lina N. Bezdetnaya, Irina Belitchenko, Alla A. Kyagova, Pascal Colosetti, Alexander Ya. Potapenko, Francois H. Guillemin

Author Affiliations +

Proceedings Volume 2972, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy VI; (1997) https://doi.org/10.1117/12.273496
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

We have studied the influence of preirradiated by visible light hematoporphyrin derivative (HpD) solution in PBS on the production of reactive oxygen species (ROS) and phagocytosis of latex particles by rat peritoneal polymorphonuclear neutrophils (PMN), and also on the delayed type hypersensitivity reaction (DTH) to sheep red blood cells in mice. The release of ROS and phagocytic activity were observed by means of registration of the luminol- enhanced chemiluminescence (ChL) in the absence and the in the presence of latex particles. Non-irradiated HpD did not influence neither spontaneous ChL response, nor latex- activated. HpD preirradiated by 135 J/m² did not affect spontaneous, but increased latex-activated ChL response by 20 percent. This fact indicates an increase in PMN phagocytic activity under the treatment with preirradiated HpD. Increase in preirradiation fluence up to 8.1 kJ/m² resulted in significant enhancement of spontaneous ChL and inhibition of latex-activated ChL response of PMN. Results of spectroscopic analysis showed negligible decease in HpD Soret band after preirradiation of HpD by the highest fluences used in this study. We could not detect any significant photoproduct formation by differential absorption spectroscopy. Earlier, we have propose the photoinduced aggragation as one of the possible mechanisms of photodegradation of aqueous porphyrin solutions. In all probability, the increase in ROS production by PMN, treated with preirradiated HpD can be attributed to the phagocytosis of aggregates formed. It is possible that ROS can influence directly the DTH-effector cells leading to the observed decrease in DTH reaction level.

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Vladislava Melnikova, Lina N. Bezdetnaya, Irina Belitchenko, Alla A. Kyagova, Pascal Colosetti, Alexander Ya. Potapenko, and Francois H. Guillemin "Increase in reactive oxygen species production and phagocytic activity of polymorphonuclear neutrophils stimulated by preirradiated hematoporphyrin-derivative solution", Proc. SPIE 2972, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy VI, (8 May 1997); https://doi.org/10.1117/12.273496

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KEYWORDS

Latex

Chemiluminescence

Photodynamic therapy

Oxygen

Particles

Spectroscopy

Blood

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