Coumarin fluorescent probes by electro-optical and laser spectrofluorimetry methods

Nicolai A. Nemkovich; Wolfram Baumann; Heribert Reis; Yurii V. Zvinevich

doi:10.1117/12.273543

7 May 1997 Coumarin fluorescent probes by electro-optical and laser spectrofluorimetry methods

Nicolai A. Nemkovich, Wolfram Baumann, Heribert Reis, Yurii V. Zvinevich

Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997) https://doi.org/10.1117/12.273543
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Modified electro-optical absorption and emission methods were used to measure the dipole moments of six coumarin fluorescent probes (CU1, CU4, CU7, CU30, CU120, CU334) in the equilibrated ground, excited Franck-Condon and equilibrated excited states. The measurements were performed in cyclohexane and dioxane at room temperature. The equilibrated ground and excited states dipole measured by electro-optical methods are compared with those derived from other measurements techniques and from semiempirical calculations. Experiments and calculations performed in this work reveal a set of anomalous and interesting properties of CU7 and CU30 in solutions. The spectral dependence of some electro-optical coefficients and, consequently, dipole moments in excited Franck-Condon state indicates that the absorption band is a superposition of several (at least two) electronic transitions. Most probably there are two close- lying singlet electronic states which contribute to the first absorption band. Our electro-optical and laser spectrofluorimetry measurements do not support the formation of twisted intramolecular charge transfer state after optical excitation in the studied coumarins, at least in cyclohexane and dioxane.

Citation Download Citation

Nicolai A. Nemkovich, Wolfram Baumann, Heribert Reis, and Yurii V. Zvinevich "Coumarin fluorescent probes by electro-optical and laser spectrofluorimetry methods", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); https://doi.org/10.1117/12.273543

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