Near-infrared Raman spectroscopy of human whole blood and serum

Andrew J. Berger; Irving Itzkan; Michael S. Feld

doi:10.1117/12.273653

2 May 1997 Near-infrared Raman spectroscopy of human whole blood and serum

Andrew J. Berger, Irving Itzkan, Michael S. Feld

Author Affiliations +

Proceedings Volume 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology; (1997) https://doi.org/10.1117/12.273653
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Optical methods of monitoring blood analytes are attractive to the medical community as alternatives to traditional chemical assays. However, the calibration process is complicated by many factors, such as overlapping spectral bands from different analytes, background noise, and interpatient variability, making the choice of measurement technique crucial. In our studies, we use near-IR Raman spectroscopy in order combine the advantages of deep penetration depths and sharp, distinct peaks. Our system consists of an 830 nm diode laser, fiber-based light delivery and collection, an f/1.8 holographic spectrograph, and a liquid nitrogen-cooled CCD detector. Blood samples from a nearby hospital are placed in cuvettes and stirred throughout the measurements to reduce heating effects. Partial least squares calibration is performed using the Raman spectra and the reference analyte concentrations from the hospital. Prediction uncertainties are calculated by cross-validation using the leave-one-out method. Using scans of fifteen minutes, we are able to extract the concentration of total protein and other analytes form serum samples with clinically acceptable levels of uncertainty. Whole blood studies are currently underway.

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Andrew J. Berger, Irving Itzkan, and Michael S. Feld "Near-infrared Raman spectroscopy of human whole blood and serum", Proc. SPIE 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology, (2 May 1997); https://doi.org/10.1117/12.273653

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