Three-dimensional limited-angle microtomography of blood cells: experimental results

Gennady G. Levin; Gennady N. Vishnyakov; Constantin S. Zakarian; Alexey V. Likhachov; Valery Vladimirov Pickalov; Gennady I. Kozinets; Julia K. Novoderzhkina; Elena A. Streletskaya

doi:10.1117/12.310549

9 June 1998 Three-dimensional limited-angle microtomography of blood cells: experimental results

Gennady G. Levin, Gennady N. Vishnyakov, Constantin S. Zakarian, Alexey V. Likhachov, Valery Vladimirov Pickalov, Gennady I. Kozinets, Julia K. Novoderzhkina, Elena A. Streletskaya

Author Affiliations +

Proceedings Volume 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V; (1998) https://doi.org/10.1117/12.310549
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Tomographic measurements of the 3D refractive index spatial distribution within optically transparent phase samples with computerized interferometric microscopes are proposed. Phase shifting interferometric microtomography applications for the 3D image reconstruction of the blood cells are represented. The immersion 100x, N.A. equals 1.25 objective was used to increase the spatial resolution and observation angle range to 90 degree. ART, combined ART and iterative Gerchberg-Papoulis 3D algorithm were used for the tomogram reconstruction. To determine the accuracy and spatial resolution of the blood cells image reconstruction by means of the interferometric microtomographic method the numerical simulations were implemented.

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Gennady G. Levin, Gennady N. Vishnyakov, Constantin S. Zakarian, Alexey V. Likhachov, Valery Vladimirov Pickalov, Gennady I. Kozinets, Julia K. Novoderzhkina, and Elena A. Streletskaya "Three-dimensional limited-angle microtomography of blood cells: experimental results", Proc. SPIE 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V, (9 June 1998); https://doi.org/10.1117/12.310549

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