Surface-enhanced Raman spectroscopic monitor of triglyceride hydrolysis in a skin pore phantom

Millicent K. Weldon; Michael D. Morris

doi:10.1117/12.345398

9 April 1999 Surface-enhanced Raman spectroscopic monitor of triglyceride hydrolysis in a skin pore phantom

Millicent K. Weldon, Michael D. Morris

Author Affiliations +

Proceedings Volume 3608, Biomedical Applications of Raman Spectroscopy; (1999) https://doi.org/10.1117/12.345398
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Bacterial hydrolysis of triglycerides is followed in a sebum probe phantom by microprobe surface-enhanced Raman scattering (SERS) spectroscopy. The phantom consists of a purpose-built syringe pump operating at physiological flow rates connected to a 300 micron i.d. capillary. We employ silicon substrate SERS microprobes to monitor the hydrolysis products. The silicon support allows some tip flexibility that makes these probes ideal for insertion into small structures. Propionibacterium acnes are immobilized on the inner surface of the capillary. These bacteria hydrolyze the triglycerides in a model sebum emulsion flowing through the capillary. The transformation is followed in vitro as changes in the SERS caused by hydrolysis of triglyceride to fatty acid. The breakdown products consists of a mixture of mono- and diglycerides and their parent long chain fatty acids. The fatty acids adsorb as their carboxylates and can be readily identified by their characteristic spectra. The technique can also confirm the presence of bacteria by detection of short chain carboxylic acids released as products of glucose fermentation during the growth cycle of these cells. Co-adsorption of propionate is observed. Spatial localization of the bacteria is obtained by ex-situ line imaging of the probe.

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Millicent K. Weldon and Michael D. Morris "Surface-enhanced Raman spectroscopic monitor of triglyceride hydrolysis in a skin pore phantom", Proc. SPIE 3608, Biomedical Applications of Raman Spectroscopy, (9 April 1999); https://doi.org/10.1117/12.345398

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