Two-dimensional fluorescence spectroscopy for online monitoring of chromatographic separation of molasses

Egbert Staerk; C. Harbeck; R. Faurie; Carsten Lindemann; Thomas-Helmut Scheper

doi:10.1117/12.372862

21 December 1999 Two-dimensional fluorescence spectroscopy for online monitoring of chromatographic separation of molasses

Egbert Staerk, C. Harbeck, R. Faurie, Carsten Lindemann, Thomas-Helmut Scheper

Author Affiliations +

Proceedings Volume 3853, Environmental Monitoring and Remediation Technologies II; (1999) https://doi.org/10.1117/12.372862
Event: Photonics East '99, 1999, Boston, MA, United States

Abstract

Monitoring and control of industrial or biotechnological processes invariably require reliable and fast analytical systems. Two-dimensional spectral fluorescence allows real time automatic measurements directly inside the process and provides a continuous stream of information compared to discrete information available from repeated sampling and offline analysis. Normally, chromatographic molasses desugarization processes are typically monitored with a combination of an online-refractometer, polarimeter and the measurement of density and conductivity. Additional information of the separation profile could be obtained by multiple sampling during the separation cycle followed by offline laboratory analysis. An optical sensor (BioView^R) allows online fluorescence measurements for a continuous monitoring directly at the outlet of the separation columns. It was the aim to predict the amino acid serine during the chromatographic cycle. Based on fluorescence, it was possible to monitor a fluorophor, which eluted a few minutes before the serine fraction during molasses desugarization. The application of fluorescence measurements for monitoring and control of chromatographic separation process may improve yield and purity of the separating fractions and can lower the costs for the next downstream processing of by-products.

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Egbert Staerk, C. Harbeck, R. Faurie, Carsten Lindemann, and Thomas-Helmut Scheper "Two-dimensional fluorescence spectroscopy for online monitoring of chromatographic separation of molasses", Proc. SPIE 3853, Environmental Monitoring and Remediation Technologies II, (21 December 1999); https://doi.org/10.1117/12.372862

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