Infuence of fiber terminal face reflection on fiber optical confocal scanning microscope

Lisong Yang; Guiying Wang; Jiangang Wang; Zhizhan Xu

doi:10.1117/12.364408

17 September 1999 Infuence of fiber terminal face reflection on fiber optical confocal scanning microscope

Lisong Yang, Guiying Wang, Jiangang Wang, Zhizhan Xu

Author Affiliations +

Proceedings Volume 3863, 1999 International Conference on Biomedical Optics; (1999) https://doi.org/10.1117/12.364408
Event: International Symposium on Biomedical Optics, 1999, Wuhan, China

Abstract

In fiber optical confocal scanning imaging system of reflection mode, interference of the reflection light beams from terminal face of the fiber coupling tip and from the surface of the sample will always destroy the image of low reflection index samples, such as CD-R pregroove basal disc and biotic samples. A quantitative analysis was given to find out the influence of fiber terminal face reflection on the system axial response. In order to avoid the influence of the fiber reflected light, the reflectivity ratio of the fiber tip to the sample should be low. Interference noise was effectively reduced by immersing the unused fiber tip into glycerol and cleaving the fiber tip end face at an angle. The proof experimental results of axial response have been shown. Finally, good quality confocal images of the recordable CD pregroove and the chromosome were presented.

Citation Download Citation

Lisong Yang, Guiying Wang, Jiangang Wang, and Zhizhan Xu "Infuence of fiber terminal face reflection on fiber optical confocal scanning microscope", Proc. SPIE 3863, 1999 International Conference on Biomedical Optics, (17 September 1999); https://doi.org/10.1117/12.364408

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