Phagocytic mechanism of live neutrophilic granulocyte with green fluorescent protein gene

Shaoqun Zeng; Weiguo Chen; Wei Zhou; Qingming Luo; Zhi Zhang

doi:10.1117/12.364343

17 September 1999 Phagocytic mechanism of live neutrophilic granulocyte with green fluorescent protein gene

Shaoqun Zeng, Weiguo Chen, Wei Zhou, Qingming Luo, Zhi Zhang

Author Affiliations +

Proceedings Volume 3863, 1999 International Conference on Biomedical Optics; (1999) https://doi.org/10.1117/12.364343
Event: International Symposium on Biomedical Optics, 1999, Wuhan, China

Abstract

The whole process of neutrophilic granulocyte phagocytosis of E.Coli BL₂₁-expressed green fluorescent protein gene was recorded with CCD coupled fluorescence microscopy in a real-time. This process contains of three stages: adhesion, ingestion, and exocytosis. The properties of each stages of phagocytosis were analyzed. The method opened new perspective in continuously observing the specific and non-specific immunity mechanism on alive cell.

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Shaoqun Zeng, Weiguo Chen, Wei Zhou, Qingming Luo, and Zhi Zhang "Phagocytic mechanism of live neutrophilic granulocyte with green fluorescent protein gene", Proc. SPIE 3863, 1999 International Conference on Biomedical Optics, (17 September 1999); https://doi.org/10.1117/12.364343

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