Random apodizated masks to improve the resolution of confocal microscopy

Yang Bu; Guiying Wang; Lisong Yang; Jiangang Wang; Zhizhan Xu

doi:10.1117/12.364407

17 September 1999 Random apodizated masks to improve the resolution of confocal microscopy

Yang Bu, Guiying Wang, Lisong Yang, Jiangang Wang, Zhizhan Xu

Author Affiliations +

Proceedings Volume 3863, 1999 International Conference on Biomedical Optics; (1999) https://doi.org/10.1117/12.364407
Event: International Symposium on Biomedical Optics, 1999, Wuhan, China

Abstract

In confocal scanning imaging system, using apodization masks can improve the system resolution. A mask based on circle grating function with different spacing is presented and made, which is simple and easy to practically make and apply. The experiments of using a mask are performed in a reflection-mode fiber confocal scanning microscope. The intensity response curves in transverse and axial direction of this system are given. A sharp straight edge of a flat object is measured. And the 2D image of a biological organism is also given. The results demonstrate that adding this apodization mask does not reduce the axial resolution while improve the transverse resolution.

Citation Download Citation

Yang Bu, Guiying Wang, Lisong Yang, Jiangang Wang, and Zhizhan Xu "Random apodizated masks to improve the resolution of confocal microscopy", Proc. SPIE 3863, 1999 International Conference on Biomedical Optics, (17 September 1999); https://doi.org/10.1117/12.364407

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