Characterization of porous polymer monoliths as flow restrictors for capillary electrophoresis on a chip

Yolanda Fintschenko; Don W. Arnold; Eric C. Peters; Frank Svec; Jean M. J. Frechet

doi:10.1117/12.359338

19 August 1999 Characterization of porous polymer monoliths as flow restrictors for capillary electrophoresis on a chip

Yolanda Fintschenko, Don W. Arnold, Eric C. Peters, Frank Svec, Jean M. J. Frechet

Author Affiliations +

Proceedings Volume 3877, Microfluidic Devices and Systems II; (1999) https://doi.org/10.1117/12.359338
Event: Symposium on Micromachining and Microfabrication, 1999, Santa Clara, CA, United States

Abstract

Capillary electrophoresis (CE) lends itself to miniaturization, because it uses electroosmotic flow rather than moving parts for flow generation. Its analytical figures of merit improve as channel dimensions decrease. However, solution flow in the small planar channels used in CE-on-a-chip is very sensitive to reservoir solution height. This adds a pressure driven flow components, which decreases resolution, sensitivity, and separation efficiency of the EOF-driven technique. We have observed that this contribution to parabolic flow from uneven solution heights can be minimized by using a porous polymer monolith (PPM) as a flow restriction plug in the reservoirs of a 75 micrometers wide X 15 micrometers deep microchannel etched in glass. Our results indicate an average PPM pore size of 1 micrometers is sufficient for flow restriction. Pore sizes below this result in charge trapping of even small dye molecules. Images of the flow profile on and off the monolith show the inverse-parabolic effect on the electroosmotic flow profile due to mismatched zeta potentials between the polymer and the fused silica wall surfaces depending on PPM surface charge and plug length.

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Yolanda Fintschenko, Don W. Arnold, Eric C. Peters, Frank Svec, and Jean M. J. Frechet "Characterization of porous polymer monoliths as flow restrictors for capillary electrophoresis on a chip", Proc. SPIE 3877, Microfluidic Devices and Systems II, (19 August 1999); https://doi.org/10.1117/12.359338

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