Direct observation of liposome uptake by leukocytes in vivo in skin blood vessels using intravital fluorescence microscopy

Jean-Marie Devoisselle; Serge R. Mordon; Sylvie Begu; Thomas Desmettre

doi:10.1117/12.384219

27 April 2000 Direct observation of liposome uptake by leukocytes in vivo in skin blood vessels using intravital fluorescence microscopy

Jean-Marie Devoisselle, Serge R. Mordon, Sylvie Begu, Thomas Desmettre

Author Affiliations +

Proceedings Volume 3921, Optical Diagnostics of Living Cells III; (2000) https://doi.org/10.1117/12.384219
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

This study aimed to observe liposome uptake by leukocytes in vivo. The study was performed on skin by using a dorsal skin-fold chamber implanted in golden hamsters using intravital microscopy. 5,6-CF-encapsulated PEGylated liposomes were injected intravenously. The skin microcirculation was observed with an intravital Eclipse E800 Nikon microscope fitted with a Xenon light source and an epi-fluorescence assembly. An ultra-high sensitivity video-camera mounted on the microscope projected the image onto a monitor, and the images were recorded for playback analysis with a digital video cassette recorder. An acute inflammatory response was obtained by removing one complete layer of skin and the underlying fascia and avascular tissue on the opposing side of the flap corresponding to an area equivalent to the window aperture. Using these model and set-up, leukocyte rolling and adhesion were easily observed and the entry of PEGylated liposomes into hamster blood leukocytes was studied for a period of 6 hours. PEGylated liposomes were clearly identified alone inside the blood flow and inside the leukocytes as soon as the inflammatory reaction appeared. This study shows for the first time that blood leukocytes in their natural milieu of whole blood are capable of interacting with, and taking up liposomes. This observation is in accordance with previous in vitro studies.

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Jean-Marie Devoisselle, Serge R. Mordon, Sylvie Begu, and Thomas Desmettre "Direct observation of liposome uptake by leukocytes in vivo in skin blood vessels using intravital fluorescence microscopy", Proc. SPIE 3921, Optical Diagnostics of Living Cells III, (27 April 2000); https://doi.org/10.1117/12.384219

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