Raman spectroscopy for the diagnosis of dysplasia in columnar and squamous epithelium

Catherine A. Kendall; Tom C. Bakker Schut; Nicholas Stone; Pela Stravroulaki; Gerwin J. Puppels; Hugh Barr M.D.

doi:10.1117/12.409321

16 November 2000 Raman spectroscopy for the diagnosis of dysplasia in columnar and squamous epithelium

Catherine A. Kendall, Tom C. Bakker Schut, Nicholas Stone, Pela Stravroulaki, Gerwin J. Puppels, Hugh Barr M.D.

Proceedings Volume 4161, Optical Biopsy and Tissue Optics; (2000) https://doi.org/10.1117/12.409321
Event: EOS/SPIE European Biomedical Optics Week, 2000, Amsterdam, Netherlands

Abstract

The incidence of oesophageal and laryngeal cancer has risen over past decades. The early detection of disease is vital for improved prognosis. The current gold standard method of tissue diagnosis is histopathology, which is invasive, costly and somewhat subjective. Raman spectroscopy however has potential for the non-invasive, early, in-vivo diagnosis of the biochemical changes associated with malignancy. Good quality Raman spectra have been measured in vitro using oesophageal and laryngeal tissue. Multivariate analysis has been implemented using principal component and linear discriminant analysis techniques. Sensitivity and specificity of more than 80% has been achieved for the discrimination of dysplasia and cancer, for both oesophageal and laryngeal tissue. On comparison with histopathology these results are seen to be an improvement, since pathology lacks sensitivity and specificity due to the subjective nature of the diagnosis. Thus illustrating that excellent discrimination between normal, dyspalstic and cancerous tissue can be achieved using Raman Spectroscopy.

Citation Download Citation

Catherine A. Kendall, Tom C. Bakker Schut, Nicholas Stone, Pela Stravroulaki, Gerwin J. Puppels, and Hugh Barr M.D. "Raman spectroscopy for the diagnosis of dysplasia in columnar and squamous epithelium", Proc. SPIE 4161, Optical Biopsy and Tissue Optics, (16 November 2000); https://doi.org/10.1117/12.409321

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available