Blood platelets: computerized morphometry applied on optical images

Farida V. Korobova; Tatyana V. Ivanova; Alexander A. Gusev; Dmitry A. Shmarov; Gennady I. Kozinets

doi:10.1117/12.407649

22 November 2000 Blood platelets: computerized morphometry applied on optical images

Farida V. Korobova, Tatyana V. Ivanova, Alexander A. Gusev, Dmitry A. Shmarov, Gennady I. Kozinets

Proceedings Volume 4163, Optical Techniques and Instrumentation for the Measurement of Blood Composition, Structure, and Dynamics; (2000) https://doi.org/10.1117/12.407649
Event: EOS/SPIE European Biomedical Optics Week, 2000, Amsterdam, Netherlands

Abstract

The new technology of computerized morphometric image analysis of platelets on blood smears was developed. In a basis of the device is included analysis of cytophotometric and morphometric parameters of platelets. Geometrical and optical parameters of platelets on 35 donors, platelet concentrates and 15 patients with haemorrhagic thrombocythaemia were investigated, average meanings for the area, diameter, its logarithms and optical density of platelets in norm were received. Distribution of the areas, diameters and optical densities of platelets of patients with haemorrhagic thrombocythaemia differed from those at the healthy people. After a course of treatment these meanings came nearer to normal. The important characteristics of platelets in platelet concentrates after three days of storage were in limits of normal meanings, but differed from those in whole blood platelets. Obtained data allow to enter the quantitative standards into investigation of platelets of the healthy people and at various alteration of thrombocytopoieses.

Citation Download Citation

Farida V. Korobova, Tatyana V. Ivanova, Alexander A. Gusev, Dmitry A. Shmarov, and Gennady I. Kozinets "Blood platelets: computerized morphometry applied on optical images", Proc. SPIE 4163, Optical Techniques and Instrumentation for the Measurement of Blood Composition, Structure, and Dynamics, (22 November 2000); https://doi.org/10.1117/12.407649

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KEYWORDS

Blood

Absorbance

Statistical analysis

Geometrical optics

Polarization

Glasses

Analytical research

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