Probing specific DNA sequences with luminescent semiconductor quantum dots

Jason R. Taylor; Shuming Nie

doi:10.1117/12.430769

18 June 2001 Probing specific DNA sequences with luminescent semiconductor quantum dots

Jason R. Taylor, Shuming Nie

Author Affiliations +

Proceedings Volume 4258, Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications; (2001) https://doi.org/10.1117/12.430769
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

The development of new fluorescent probes has impacted many areas of research such as medical diagnostics, high-speed drug screening, and basic molecular biology. Main limitations to traditional organic fluorophores are their relatively weak intensities, short life times (eg., photobleaching), and broad emission spectra. The desire for more intense fluorescent probes with higher quality photostability and narrow emission wavelengths has led to the development and utilization of semiconductor quantum dots as a new label. In this work, we have modified semicondutor quantum dots (QD's) with synthetic oligonucleotides to probe a specific DNA target sequence both in solution as well as immobilized on a solid substrate. In the first approach, specific target sequences are detected in solution by using short oligonucleotide probes, which are covalently linked to semiconductor quantum dots. In the second approach, DNA target sequences are covalently attached to a glass substrate and detected using oligonucleotides linked to semiconductor quantum dots.

Citation Download Citation

Jason R. Taylor and Shuming Nie "Probing specific DNA sequences with luminescent semiconductor quantum dots", Proc. SPIE 4258, Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications, (18 June 2001); https://doi.org/10.1117/12.430769

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