Digital imaging of diabetic endothelial cells

Jolanta Paltauf-Doburzynska; Sepp D. Kohlwein; Wolfgang F. Graier

doi:10.1117/12.426756

10 May 2001 Digital imaging of diabetic endothelial cells

Jolanta Paltauf-Doburzynska, Sepp D. Kohlwein, Wolfgang F. Graier

Proceedings Volume 4260, Optical Diagnostics of Living Cells IV; (2001) https://doi.org/10.1117/12.426756
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Endothelial cells release factors that regulate dilatation and contraction of the vessels. They play an important role in modulating both the inflammatory response and vasomotor abnormalities that occur in coronary artery diseases. This endothelial function is associated with changes of intracellular Ca²⁺ concentration. For this study we used spatially and temporally resolved measurements of local Ca²⁺ concentration in human endothelial cells cultured in high glucose containing medium. Deconvolution techniques procedure allowed determination of intracellular Ca²⁺ concentration and its distribution into cellular compartments. We also used a confocal microscope for visualization of intracellular compartments (endoplasmatic reticulum, mitochondria) under normal and pathological conditions. We showed that the interrupted connection between superficial compartments and membrane channels is already the beginning of the cell damage in diabetes.

Citation Download Citation

Jolanta Paltauf-Doburzynska, Sepp D. Kohlwein, and Wolfgang F. Graier "Digital imaging of diabetic endothelial cells", Proc. SPIE 4260, Optical Diagnostics of Living Cells IV, (10 May 2001); https://doi.org/10.1117/12.426756

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