Detection of pathogens using on-chip electrochemical analysis of PCR amplified DNA molecules

Dalibor Hodko; Lindsay Raymer; Stephanie M. Herbst; James W. Magnuson; David Gaskin

doi:10.1117/12.427958

21 May 2001 Detection of pathogens using on-chip electrochemical analysis of PCR amplified DNA molecules

Dalibor Hodko, Lindsay Raymer, Stephanie M. Herbst, James W. Magnuson, David Gaskin

Proceedings Volume 4265, Biomedical Instrumentation Based on Micro- and Nanotechnology; (2001) https://doi.org/10.1117/12.427958
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

The sensitivity and speed of methods for the detection of microorganisms and/or cells need to be constantly improved to provide timely and accurate analysis in large number of important applications. Such applications range from detection of pathogens in drinking water, biological warfare agents, biomedical diagnostics and food industry. The trends toward miniaturization of sensors using microfluidic and nanofluidic on-chip devices will push current detection limits to lower concentrations than what is offered by the present analytical equipment and/or detection kids. Microfluidic devices have been used to perform DNA analysis, polymerase chain reaction analysis, capillary electrophoresis and hybridization to oligonucleotide probes. This paper describes a new approach for the detection of pathogens on contaminated surfaces, which will integrated sampling, concentration and detection of targeted microorganisms.

Citation Download Citation

Dalibor Hodko, Lindsay Raymer, Stephanie M. Herbst, James W. Magnuson, and David Gaskin "Detection of pathogens using on-chip electrochemical analysis of PCR amplified DNA molecules", Proc. SPIE 4265, Biomedical Instrumentation Based on Micro- and Nanotechnology, (21 May 2001); https://doi.org/10.1117/12.427958

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