Antidiabetics and diuretics show phototoxicity in HaCaT cells

Edgar Selvaag M.D.; Anita B. Petersen; Robert Gniadecki; Tine Thorn; Hans Christian Wulf

doi:10.1117/12.446520

26 October 2001 Antidiabetics and diuretics show phototoxicity in HaCaT cells

Edgar Selvaag M.D., Anita B. Petersen, Robert Gniadecki, Tine Thorn, Hans Christian Wulf

Proceedings Volume 4433, Laser-Tissue Interactions, Therapeutic Applications, and Photodynamic Therapy; (2001) https://doi.org/10.1117/12.446520
Event: European Conferences on Biomedical Optics, 2001, Munich, Germany

Abstract

The antidiabetics tolbutamide, glibenclamide, and glipizide, and the diuretics bendroflumethiazide, butizide, furosemide, hydrochlorothiazide, and trichlormethiazide were investigated for potential phototoxicity in the HaCaT cell line. The cells were incubated with the drugs and then exposed to UVA1 irradiation. The effects of the antioxidants L-ascorbic acid, and (alpha) -tocopherol on oxidative DNA damage were assessed. Bendroflumethiazide, furosemide, hydrochlorothiazide, trichlormethiazide, or tolbutamide induced dose-dependent phototoxicity. Cells incubated with bendroflumethiazide, tolbutamide, and glibenclamide, and irradiated with UVA1 demonstrated an increased oxidative DNA damage. Pre-treatment with L-ascorbic acid, or (alpha) -tocopherol, suppressed the UVA-induced DNA damage in cells incubated with 1 mM of bendroflumethiazide, furosemide, glibenclamide, glipizide, tolbutamide, and trichloromethiazide, further implying the involvement of reactive oxygen species in the phototoxic DNA damage. These results may indicate a link between phototoxic and photocancerogenic potential of the sulfonamide-derived oral antidiabetic and diuretic drugs, as it has previously been recognized for psoralen, chlorpromazine, and fluoroquinolones. Excessive exposure to UV light may be deleterious for patients treated with these drugs.

Citation Download Citation

Edgar Selvaag M.D., Anita B. Petersen, Robert Gniadecki, Tine Thorn, and Hans Christian Wulf "Antidiabetics and diuretics show phototoxicity in HaCaT cells", Proc. SPIE 4433, Laser-Tissue Interactions, Therapeutic Applications, and Photodynamic Therapy, (26 October 2001); https://doi.org/10.1117/12.446520

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available