Kinetic of antigent-antibody reactions with scattering method

Olexander I. Bilyi; Yevgen M. Kiselyov; Volodymyr P. Novikov

doi:10.1117/12.432972

11 July 2001 Kinetic of antigent-antibody reactions with scattering method

Olexander I. Bilyi, Yevgen M. Kiselyov, Volodymyr P. Novikov

Proceedings Volume 4515, Light and Optics in Biomedicine; (2001) https://doi.org/10.1117/12.432972
Event: Light and Optics in Biomedicine, 2000, Warsaw, Poland

Abstract

The immune reactions of interaction antigen-antibody represent specific effect of an antigene with an antibody, which outcome are the complex immune aggregates forming precipitate in case of a soluble antigene, or agglutinate in case of a corpuscular antigene. Immunological methods which uses in the quality of carrier protein latex's polymeric microspheresis, gained name and method latex agglutination. Polymeric microspheresis have the array of advantages before biological carries, which consist in the opportunity of the variation of attributes surface and size microspheresis in the broad band of meanings with the preservation of narrow distribution particles behind measurements, the putting of functional groups, necessary for bunch with ligand on stage their synthesis, in ragidity at storage. The quantitative evaluation of parameters of a response of interaction antigen-antibody in immunology is possible by optical methods on a measurement of a modification of intensity of a light stream of a solution in an outcome of a course of a reaction. Concentration of immune complexes determine both on slacking a taking place stream of light, and on a modification of intensity of a stream of light scattering suspended particles in a solution. The process light scattering by colloidal aggregates are formed from suspension microspheresis with adsorbed on their surface protein is described. In report the physics principle of registration immune reaction by light scattering methods is concerned. The results of the effectiveness latex's preparation created on basis of the polymeric carries is described.

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Olexander I. Bilyi, Yevgen M. Kiselyov, and Volodymyr P. Novikov "Kinetic of antigent-antibody reactions with scattering method", Proc. SPIE 4515, Light and Optics in Biomedicine, (11 July 2001); https://doi.org/10.1117/12.432972

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KEYWORDS

Particles

Latex

Polymers

Light scattering

Proteins

Scattering

Polymerization

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