Antimicrobial effect of low-level laser therapy in the presence of photosensitizer on human saliva bacteria

Iriana Carla J. Zanin; Aldo Brugnera Jr.; Jose Francisco Hoefling; Fatima A. A. Zanin D.D.S.; Reginaldo Bruno Goncalves

doi:10.1117/12.469319

3 June 2002 Antimicrobial effect of low-level laser therapy in the presence of photosensitizer on human saliva bacteria

Iriana Carla J. Zanin, Aldo Brugnera Jr., Jose Francisco Hoefling, Fatima A. A. Zanin D.D.S., Reginaldo Bruno Goncalves

Proceedings Volume 4610, Lasers in Dentistry VIII; (2002) https://doi.org/10.1117/12.469319
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

The purpose of this in vitro study was to determine whether low-level laser light in the presence of a photosensitizer can kill streptococci in human saliva. Samples of stimulated saliva were collected from ten healthy subjects. These samples were mixed and exposed to a gallium-aluminium-arsenide laser light (660 nm) in the presence of toluidine blue O. Viable total streptococci and mutans streptococci were counted on plates with MSA and MSB, respectively, following incubation at 37 degree(s)C, 10% CO₂ for 48 hours. The exposure of saliva to laser light in the absence of the dye, or the dye in the absence of the laser light showed no significant effect on the viability of the microorganisms. A decrease in the number of viable microorganisms was only verified when they were exposed to both the laser light and the dye at the same time. A total inhibition growth of the mutans streptococci and a substantial killing rate of total streptococci were achieved with a dye concentration of 100 mg/mL and an energy density of 28.8 J/cm². These results imply that total streptococci and mutans streptococci present in human saliva can be killed by low-level laser light in the presence of toluidine blue O.

Citation Download Citation

Iriana Carla J. Zanin, Aldo Brugnera Jr., Jose Francisco Hoefling, Fatima A. A. Zanin D.D.S., and Reginaldo Bruno Goncalves "Antimicrobial effect of low-level laser therapy in the presence of photosensitizer on human saliva bacteria", Proc. SPIE 4610, Lasers in Dentistry VIII, (3 June 2002); https://doi.org/10.1117/12.469319

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available