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We have presented experimental data on in vivo and in vitro study on the optical clearing of the scleral tissue controlled by administration of the osmotic liquids. For example, administration of glucose induces matter diffusion and as a result equalization of the refractive indices of scleral tissue scatterers and interstitial fluid. The significant increase of transmittance and decrease of reflectance of the sclera under glucose solution action have been demonstrated. The glucose diffusion coefficient has been estimated. The results of in vitro study of polarization features of scleral optical transmittance at tissue clearing are also presented.
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Valery V. Tuchin, Alexey N. Bashkatov, Elina A. Genina, Yurii P. Sinichkin, "Scleral tissue clearing effects," Proc. SPIE 4611, Ophthalmic Technologies XII, (13 June 2002); https://doi.org/10.1117/12.470606