Paper
5 June 2002 Knowledge-based image processing for on-off type DNA microarray
Jong Dae Kim, Seo Kyu Kim, Jeong Sik Cho, Jongwon Kim
Author Affiliations +
Abstract
This paper addresses the image processing technique for discriminating whether the probes are hybrized with target DNA in the Human Papilloma Virus (HPV) DNA Chip designed for genotyping HPV. In addition to the probes, the HPV DNA chip has markers that always react with the sample DNA. The positions of probe-dots in the final scanned image are fixed relative to the marker-dot locations with a small variation according to the accuracy of the dotter and the scanner. The probes are duplicated 4 times for the diagnostic stability. The prior knowledges such as the maker relative distance and the duplication information of probes is integrated into the template matching technique with the normalized correlation measure. Results show that the employment of both of the prior knowledges is to simply average the template matching measures over the positions of the markers and probes. The eventual proposed scheme yields stable marker locating and probe classification.
© (2002) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jong Dae Kim, Seo Kyu Kim, Jeong Sik Cho, and Jongwon Kim "Knowledge-based image processing for on-off type DNA microarray", Proc. SPIE 4623, Functional Monitoring and Drug-Tissue Interaction, (5 June 2002); https://doi.org/10.1117/12.469458
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CITATIONS
Cited by 5 scholarly publications.
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KEYWORDS
Image processing

Diagnostics

Scanners

Binary data

Solids

Distance measurement

Image segmentation

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