Surface-enhanced Raman substrate optimization for bacterial identification

Jay Pendell Jones; Nicholas F. Fell Jr.; Troy A. Alexander; Kristl Dorschner; Christin Tombrello; B. Ritz Reis; Augustus W. Fountain III

doi:10.1117/12.486987

22 September 2003 Surface-enhanced Raman substrate optimization for bacterial identification

Jay Pendell Jones, Nicholas F. Fell Jr., Troy A. Alexander, Kristl Dorschner, Christin Tombrello, B. Ritz Reis, Augustus W. Fountain III

Author Affiliations +

Proceedings Volume 5071, Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Defense and Law Enforcement II; (2003) https://doi.org/10.1117/12.486987
Event: AeroSense 2003, 2003, Orlando, Florida, United States

Abstract

The threat of biological agents to soldiers and the civilian community was amply demonstrated in the fall of 2001. We are examining the feasibility of using surface-enhanced Raman spectroscopy (SERS) to detect and identify bacteria. In order to use SERS for bacterial detection and identification, it is necessary to determine the most appropriate type of SERS substrate to use. We are examining gold colloids in suspension, immobilized gold colloids, electrochemically roughened gold, periodic particle arrays (PPA), and film over nanosphere substrates (FONS). Briefly, PPA’s are prepared by depositing gold or silver in the interstitial spaces in a close-packed array of polystyrene nanospheres, while FONS are prepared by depositing approximately half a nanosphere diameter of gold or silver on top of a close-packed array of polymer nanospheres. We are evaluating each of these substrate types to determine which will have a high affinity for bacteria, whether we need to modify the surface of the substrate to attract bacteria, and the degree to which each type of substrate enhances the Raman scattering from the bacterial targets. We will present the results of our initial evaluations of substrates and the spectra obtained for several species of bacteria.

Citation Download Citation

Jay Pendell Jones, Nicholas F. Fell Jr., Troy A. Alexander, Kristl Dorschner, Christin Tombrello, B. Ritz Reis, and Augustus W. Fountain III "Surface-enhanced Raman substrate optimization for bacterial identification", Proc. SPIE 5071, Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Defense and Law Enforcement II, (22 September 2003); https://doi.org/10.1117/12.486987

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE