An integrated software solution for real-time PCR analysis based on microfluidic biochip

Qinghui Wang; Haiqing Gong

doi:10.1117/12.498280

18 April 2003 An integrated software solution for real-time PCR analysis based on microfluidic biochip

Qinghui Wang, Haiqing Gong

Author Affiliations +

Proceedings Volume 5119, Bioengineered and Bioinspired Systems; (2003) https://doi.org/10.1117/12.498280
Event: Microtechnologies for the New Millennium 2003, 2003, Maspalomas, Gran Canaria, Canary Islands, Spain

Abstract

In this paper, we present an integrated and automated prototype system which has been developed for real-time polymerase chain reaction (PCR) analysis based on microfluidic PCR array chips. The system integrates the PCR thermal cycling and optical detection capabilities to enable real-time fluorescence imaging and image processing for data analysis. The main advantage of the system is that it provides a solution that can rapidly perform and evaluate PCR experiment simultaneously on microfluidic PCR array chips. The system has demonstrated fast and efficient on-chip real-time PCR analysis using human genomic DNA samples. The implementation of the system integration is a multi-thread Windows software with component structure which is written in Visual C++.

Citation Download Citation

Qinghui Wang and Haiqing Gong "An integrated software solution for real-time PCR analysis based on microfluidic biochip", Proc. SPIE 5119, Bioengineered and Bioinspired Systems, (18 April 2003); https://doi.org/10.1117/12.498280

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