Multiphoton imaging of cardiovascular structures

Katja Schenke-Layland; Florian Opitz; Iris Riemann; Ulrich A. Stock; Karsten Konig

doi:10.1117/12.545882

8 September 2004 Multiphoton imaging of cardiovascular structures

Katja Schenke-Layland, Florian Opitz, Iris Riemann, Ulrich A. Stock, Karsten Konig

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Proceedings Volume 5463, Femtosecond Laser Applications in Biology; (2004) https://doi.org/10.1117/12.545882
Event: Photonics Europe, 2004, Strasbourg, France

Abstract

Near infrared (NIR) femtosecond laser imaging systems represent a novel and very promising diagnostic technology for non-invasive cross-sectional analysis of living biological tissues. In this study 3D multiphoton imaging with submicron resolution has been performed for non-invasive analysis of living native and tissue-engineered (TE) heart valves and blood vessels. High-resolution autofluorescence and second harmonic generation (SHG) images of collagenous structures and elastic fibers were demonstrated using multiphoton excitation at two different wavelengths. Non-invasive optical sections have been obtained without the need of staining or embedding. The quality of the resulting three-dimensional images allowed exact differentiation between collagenous structures and elastic fibers. These experimental results are very encouraging for NIR femtosecond laser scanning microscopy as a useful tool for future non-destructive monitoring and characterization of vital and intact TE cardiovascular structures.

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Katja Schenke-Layland, Florian Opitz, Iris Riemann, Ulrich A. Stock, and Karsten Konig "Multiphoton imaging of cardiovascular structures", Proc. SPIE 5463, Femtosecond Laser Applications in Biology, (8 September 2004); https://doi.org/10.1117/12.545882

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KEYWORDS

Heart

Collagen

Tissues

Blood vessels

Near infrared

Femtosecond phenomena

Imaging systems

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