Multiphoton nanosurgery in cells and tissues

Iris Riemann; Tiemo Anhut; Frank Stracke; Ronan Le Harzic; Karsten Koenig

doi:10.1117/12.590487

15 April 2005 Multiphoton nanosurgery in cells and tissues

Iris Riemann, Tiemo Anhut, Frank Stracke, Ronan Le Harzic, Karsten Koenig

Proceedings Volume 5695, Optical Interactions with Tissue and Cells XVI; (2005) https://doi.org/10.1117/12.590487
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Multiphoton Microscopy with a femtosecond pulsed Ti:sapphire laser in the near infrared (NIR) enables the user not only to image cells and tissues with a subcellular resolution but also to perform highly precise nanosurgery. Intratissue compartments, single cells and even cell organelles like mitochondria, membranes or chromosomes can be manipulated and optically knocked out. Working at transient TW/cm² laser intensities, single cells of tumor-sphaeroids were eliminated efficiently inside the sphaeroid without damaging the neighbour cells. Also single organelles of cells inside tissues could be optically knocked out with the nanoscalpel without collateral damage. Tissue structures inside a human tooth have been ablated with sizes below 1 μm. This method may become a useful instrument for nano-manipulating and surgery in several fields of science, including targeted transfection.

Citation Download Citation

Iris Riemann, Tiemo Anhut, Frank Stracke, Ronan Le Harzic, and Karsten Koenig "Multiphoton nanosurgery in cells and tissues", Proc. SPIE 5695, Optical Interactions with Tissue and Cells XVI, (15 April 2005); https://doi.org/10.1117/12.590487

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