High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera

Sumin Han; Kang-Bin Im; Hwajoon Park; Dongsun Kim; Beop-Min Kim

doi:10.1117/12.591465

24 March 2005 High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera

Sumin Han, Kang-Bin Im, Hwajoon Park, Dongsun Kim, Beop-Min Kim

Author Affiliations +

Proceedings Volume 5701, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XII; (2005) https://doi.org/10.1117/12.591465
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Using an acousto-optic beam deflector and a line scan camera, we constructed a non-mechanical, slit-scanning confocal microscope. It generates two-dimensional 512x512 images with speed of 60 frames/sec, which can be easily expanded, to 100 frames/sec. The measured axial and lateral resolutions of the system are 3.3 mm and <1 mm, respectively, with a 50X objective. This simple design can produce frames rates faster than other commercial products with comparable resolution, which may be useful for analysis of rapid interactions in various biological applications.

Citation Download Citation

Sumin Han, Kang-Bin Im, Hwajoon Park, Dongsun Kim, and Beop-Min Kim "High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera", Proc. SPIE 5701, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XII, (24 March 2005); https://doi.org/10.1117/12.591465

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