Enzymatic digestion and liquid chromatography in micropillar reactors: hydrodynamic versus electro-osmotic flow

Nicolas Sarrut; Stephanie Bouffet; Frederique Mittler; Olivier Constantin; P. Combette; Jan Sudor; Florence Ricoul; Francoise Vinet; Jerome Garin; Claude Vauchier

doi:10.1117/12.590150

22 January 2005 Enzymatic digestion and liquid chromatography in micropillar reactors: hydrodynamic versus electro-osmotic flow

Nicolas Sarrut, Stephanie Bouffet, Frederique Mittler, Olivier Constantin, P. Combette, Jan Sudor, Florence Ricoul, Francoise Vinet, Jerome Garin, Claude Vauchier

Author Affiliations +

Proceedings Volume 5718, Microfluidics, BioMEMS, and Medical Microsystems III; (2005) https://doi.org/10.1117/12.590150
Event: MOEMS-MEMS Micro and Nanofabrication, 2005, San Jose, California, United States

Abstract

Enzymatic digestion and peptide separation are basic steps for preparation of protein samples prior to their analysis by mass spectrometry. Micro-pillar reactors for digestion and reverse phase liquid chromatography were designed and constructed using semiconductor technologies. The performances of the micro-machined reactors were evaluated: complete Cytochrome C digestion was achieved in 6 min for a concentration up to 25 pmol.μl^-1 and the separation micro-column was seen to exhibit separation capabilities and capacity close to those obtained with a commercial column. Furthermore, a comparative study between hydrodynamic and electroosmotic driven flows was performed for each peptide of a Cytochrome C digest. It was demonstrated that parasitic electrophoretic phenomena disturbed peptide mobility but not protein identification.

Citation Download Citation

Nicolas Sarrut, Stephanie Bouffet, Frederique Mittler, Olivier Constantin, P. Combette, Jan Sudor, Florence Ricoul, Francoise Vinet, Jerome Garin, and Claude Vauchier "Enzymatic digestion and liquid chromatography in micropillar reactors: hydrodynamic versus electro-osmotic flow", Proc. SPIE 5718, Microfluidics, BioMEMS, and Medical Microsystems III, (22 January 2005); https://doi.org/10.1117/12.590150

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available