Influence of low level laser radiation on migration of stem cells

Levon V. Gasparyan M.D.; Grigory Brill; Anu M. Makela M.D.

doi:10.1117/12.660042

25 October 2005 Influence of low level laser radiation on migration of stem cells

Levon V. Gasparyan M.D., Grigory Brill, Anu M. Makela M.D.

Author Affiliations +

Proceedings Volume 5968, Laser Florence 2004: A Window on the Laser Medicine World; 596808 (2005) https://doi.org/10.1117/12.660042
Event: Laser Florence 2004, 2004, Florence, Italy

Abstract

The long-term effects of low level laser therapy can involve mechanisms connected with activation of migration of stem cells towards damaged areas. Stromal cell-derived factor-1 alpha (SDF-1) plays a critical role in stem cell migration towards areas of tissue injury and hypoxia. This study examines the influence of laser light on migration of stem cells (FDCP-mix stem cell line) in absence and in presence of SDF-1, using Transwell system with 2 connected chambers separated by a filter. The first chamber contained medium and stem cells, second chamber contained medium with or without SDF. Group 1 cells served as a control. Cells of groups 2 and 5 were irradiated by red diode laser (660 nm, 20 mW, 15 min). Cells of groups 3 and 6 received infrared (IR) diode laser (958 nm, 36 mW, 15 min). Group 4 was SDF-1 control. Stem cells were seeded into first chamber of the Transwell system, and filters were placed into the wells containing medium with 150 ng/ml SDF-1 (groups 4,5,6) or without SDF-1 (groups 1,2,3). After incubation cells were collected and counted. The count of spontaneously migrated cells was 1496,5±409 (100%) in case of control. Red and IR laser light increased motility of stem cells up to 1892±283 (126%) and 2255,5±510 (151%) accordingly. Influence of SDF-1 caused directed migration of 3365,5±489 cells (225%). Combined effects of light irradiation and SDF-1 were significantly stronger (p<0,05): 5813±1199 (388%) for SDF-1 and red laser , 6391,5±540 (427%) for SDF-1 and IR laser. Study shows that laser radiation can up-regulate stem cell migration towards higher SDF-1 gradient.

Citation Download Citation

Levon V. Gasparyan M.D., Grigory Brill, and Anu M. Makela M.D. "Influence of low level laser radiation on migration of stem cells", Proc. SPIE 5968, Laser Florence 2004: A Window on the Laser Medicine World, 596808 (25 October 2005); https://doi.org/10.1117/12.660042

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE