Three-dimensional measurement of microorganism by retardation modulated differential interference contrast microscope

G. Aoki; M. Itoh; Y. Yasuno; T. Yatagai

doi:10.1117/12.645962

23 February 2006 Three-dimensional measurement of microorganism by retardation modulated differential interference contrast microscope

G. Aoki, M. Itoh, Y. Yasuno, T. Yatagai

Author Affiliations +

Proceedings Volume 6090, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII; 60900A (2006) https://doi.org/10.1117/12.645962
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

We propose a new technique for obtaining three-dimensional phase distribution on differential interference contrast microscope to modulate relative phase retardation between two shear beams. Using partial coherent theory we extract the phase information from two different retardation images. For the object in a weak phase region, simple formula is derived. The images of nematomorph were obtained in vivo.

Citation Download Citation

G. Aoki, M. Itoh, Y. Yasuno, and T. Yatagai "Three-dimensional measurement of microorganism by retardation modulated differential interference contrast microscope", Proc. SPIE 6090, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII, 60900A (23 February 2006); https://doi.org/10.1117/12.645962

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