Bacteria-clay interactions investigated by light scattering and phase contrast microscopy

Alexandra Alimova; Karin Block; Elizabeth Rudolph; A. Katz; J. C. Steiner; P. Gottlieb; R. R. Alfano

doi:10.1117/12.648042

27 February 2006 Bacteria-clay interactions investigated by light scattering and phase contrast microscopy

Alexandra Alimova, Karin Block, Elizabeth Rudolph, A. Katz, J. C. Steiner, P. Gottlieb, R. R. Alfano

Author Affiliations +

Proceedings Volume 6094, Optical Diagnostics and Sensing VI; 60940E (2006) https://doi.org/10.1117/12.648042
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Light scattering experiments and phase contrast microscopy are used to evaluate the aggregate-forming characteristics of simple clay-bacteria mixtures. Colloidal suspensions of negatively charged Pseudomonas syringae (Ps) and Mg²⁺-, Li⁺- or Ca²⁺ -exchanged smectite (and non-exchanged smectite) are flocculated in neutral (pH 7) aqueous media. Aggregate formation is monitored using changes in optical transmission. Clustering is observed in all the clay-bacteria preparations. The Li⁺-substituted clay aggregates average 50-300 microns in diameter, in contrast to the Ca²⁺- substituted clay that produces aggregates of 10-50 microns in diameter. Light scattering measurements indicate that aggregates begin forming 3 hours after mixing and that the (larger sized) aggregates exhibit less scattering than a mixture with an equivalent concentration of unattached Ps and clay particles.

Citation Download Citation

Alexandra Alimova, Karin Block, Elizabeth Rudolph, A. Katz, J. C. Steiner, P. Gottlieb, and R. R. Alfano "Bacteria-clay interactions investigated by light scattering and phase contrast microscopy", Proc. SPIE 6094, Optical Diagnostics and Sensing VI, 60940E (27 February 2006); https://doi.org/10.1117/12.648042

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