Optical fiber direct-sensing biosensor applied in detecting biolayer thickness of nanometer grade

Yan Sun; Mingming Li; Hong Zhao; Yu Xiao Yang; Lu Zhang

doi:10.1117/12.678575

23 February 2006 Optical fiber direct-sensing biosensor applied in detecting biolayer thickness of nanometer grade

Yan Sun, Mingming Li, Hong Zhao, Yu Xiao Yang, Lu Zhang

Author Affiliations +

Proceedings Volume 6150, 2nd International Symposium on Advanced Optical Manufacturing and Testing Technologies: Optical Test and Measurement Technology and Equipment; 61502Z (2006) https://doi.org/10.1117/12.678575
Event: 2nd International Symposium on Advanced Optical Manufacturing and Testing Technologies, 2005, Xian, China

Abstract

An optical fiber biosensor is introduced herein, which could directly detect biological interaction such as immunoreactions of antigens and antibodies without destroy the biolayer. The test is based on the theory of multilayer-reflection principle in white-light interferometry. When immunoreactions occur, the reflected spectrum phase shifts. Immunoreactions could be detected by means of reflected spectrum phase shifting, or by biolayer thickness changing. Continuously detecting of thickness changing on a fractional nanometer scale with subsecond repetition times is allowed in this system. The detecting system has high sensitivity, high precision, high speed, cost effective and working on a high reliability. The bioprobe is easy integrated as a BlAcore. The system and the experimental results on the reaction of rabbit-IgG with anti-rabbit-IgG are described in this paper. A sandwich method was adopted in the experiments.

Citation Download Citation

Yan Sun, Mingming Li, Hong Zhao, Yu Xiao Yang, and Lu Zhang "Optical fiber direct-sensing biosensor applied in detecting biolayer thickness of nanometer grade", Proc. SPIE 6150, 2nd International Symposium on Advanced Optical Manufacturing and Testing Technologies: Optical Test and Measurement Technology and Equipment, 61502Z (23 February 2006); https://doi.org/10.1117/12.678575

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