Oxytetracycline analysis in honey using a specific portable analyzer

Guoying Chen; Daniel Schwartz; S. Braden; Alberto Nunez

doi:10.1117/12.735029

22 October 2007 Oxytetracycline analysis in honey using a specific portable analyzer

Guoying Chen, Daniel Schwartz, S. Braden, Alberto Nunez

Author Affiliations +

Proceedings Volume 6761, Optics for Natural Resources, Agriculture, and Foods II; 67610K (2007) https://doi.org/10.1117/12.735029
Event: Optics East, 2007, Boston, MA, United States

Abstract

Oxytetracycline (OTC) residue in honey is detected using a portable analyzer designed to specifically target tetracycline (TC) drugs based on europium-sensitized luminescence (ESL). A 385 nm light emitting diode (LED) is used as the excitation source and a photomultiplier tube as the light detector. OTC is extracted from honey and cleaned up by solid phase extraction (SPE) using Strata X-WC weak cation exchange cartridges. To the eluate Eu(III) is added to form a Eu-TC chelate at pH 8.5. Efficient intrachelate energy transfer allows sensitive OTC detection at λ_ex=385 nm and λ_em=610 nm. After a 25-µs time delay, the ESL signal is integrated over a 25-1000 µs interval. The signal intensity reveals a linear relationship (R²=0.972) to OTC concentrations in the 10-200 ng/g range. The limit-of-detection is 6.7 ng/g with an average 5.8% relative standard deviation. The background signal corresponds to ~10 ppb. This instrumentation and method combination enables field analysis that is especially useful for beekeeping industry.

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Guoying Chen, Daniel Schwartz, S. Braden, and Alberto Nunez "Oxytetracycline analysis in honey using a specific portable analyzer", Proc. SPIE 6761, Optics for Natural Resources, Agriculture, and Foods II, 67610K (22 October 2007); https://doi.org/10.1117/12.735029

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