Applying adaptive optics to three-dimensional wide-field microscopy

Peter Kner; John Sedat; David Agard; Zvi Kam

doi:10.1117/12.773731

8 February 2008 Applying adaptive optics to three-dimensional wide-field microscopy

Peter Kner, John Sedat, David Agard, Zvi Kam

Proceedings Volume 6888, MEMS Adaptive Optics II; 688809 (2008) https://doi.org/10.1117/12.773731
Event: MOEMS-MEMS 2008 Micro and Nanofabrication, 2008, San Jose, California, United States

Abstract

Depth aberrations are a major source of image degradation in three-dimensional microscopy, causing a significant loss of resolution and intensity deep into the sample. These aberrations occur because of an inevitable mismatch between the sample refractive index and the immersion medium index. We have built a wide-field fluorescence microscope that incorporates a large-throw deformable mirror to correct for depth aberrations in 3D imaging. We demonstrate a corrected point spread function imaging beads in water with an oil immersion lens and a twofold improvement in peak signal intensity. We apply this new microscope to imaging biological samples, and show sharper images and improved deconvolution.

Citation Download Citation

Peter Kner, John Sedat, David Agard, and Zvi Kam "Applying adaptive optics to three-dimensional wide-field microscopy", Proc. SPIE 6888, MEMS Adaptive Optics II, 688809 (8 February 2008); https://doi.org/10.1117/12.773731

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