Obstacles to the production of protein microarray cassettes

Jean Montagu; Herman DeWeerd; Nathan Tyburczy; Natalia Rodionova; Peter Maimonis

doi:10.1117/12.806639

23 February 2009 Obstacles to the production of protein microarray cassettes

Jean Montagu, Herman DeWeerd, Nathan Tyburczy, Natalia Rodionova, Peter Maimonis

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Proceedings Volume 7207, Microfluidics, BioMEMS, and Medical Microsystems VII; 72070F (2009) https://doi.org/10.1117/12.806639
Event: SPIE MOEMS-MEMS: Micro- and Nanofabrication, 2009, San Jose, California, United States

Abstract

This paper examines the necessary technologies to be mastered in order to build a practical micro array-based immunoassay cassette and its processing station for protein analysis. The interdependence of surface-chemistry, dye stability and imaging are outlined showning why a treated 100 nm film of Nitrocellulose adhered by an intervening layer to glass offers an efficacious surface for immobilizing an array of protein probes. The properties of a storage surface to support in desiccated form, fluidize and transport additional reagents are outlined and a practical solution proposed. Wet and Dry imaging are compared. The steps and functions expected for an assay platform comprising processing station and biochip cassette are identified. The performance of a successful bench-top automated multiplex immunoassay system is briefly described

Citation Download Citation

Jean Montagu, Herman DeWeerd, Nathan Tyburczy, Natalia Rodionova, and Peter Maimonis "Obstacles to the production of protein microarray cassettes", Proc. SPIE 7207, Microfluidics, BioMEMS, and Medical Microsystems VII, 72070F (23 February 2009); https://doi.org/10.1117/12.806639

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KEYWORDS

Proteins

Microfluidics

Molecules

Diffusion

Glasses

Control systems

Microfluidic imaging

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