Single molecule observations of DNA hybridization kinetics

Ana Jofre; Jason Case; Sean Hicks

doi:10.1117/12.828027

20 August 2009 Single molecule observations of DNA hybridization kinetics

Ana Jofre, Jason Case, Sean Hicks

Author Affiliations +

Proceedings Volume 7400, Optical Trapping and Optical Micromanipulation VI; 740014 (2009) https://doi.org/10.1117/12.828027
Event: SPIE NanoScience + Engineering, 2009, San Diego, California, United States

Abstract

Two 25 base-pair complementary DNA strands are encapsulated within an optically trapped nano-droplet, and we observe the kinetics of their hybridization in dynamic equilibrium via single molecule fluorescence resonance energy transfer (FRET) as a function of temperature and of the solution's NaCl concentration. We have observed binding and unbinding events between the two freely diffusing DNA strands, and our measurements reveal that the duplex can exist in multiple conformational states at elevated temperatures and low concentrations of NaCl.

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Ana Jofre, Jason Case, and Sean Hicks "Single molecule observations of DNA hybridization kinetics", Proc. SPIE 7400, Optical Trapping and Optical Micromanipulation VI, 740014 (20 August 2009); https://doi.org/10.1117/12.828027

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