Nano-engineering of materials for nonlinear optical imaging

Koen Clays; Jos Vanderleyden; Harry L Anderson

doi:10.1117/12.824517

20 August 2009 Nano-engineering of materials for nonlinear optical imaging

Koen Clays, Jos Vanderleyden, Harry L Anderson

Author Affiliations +

Proceedings Volume 7413, Linear and Nonlinear Optics of Organic Materials IX; 74130J (2009) https://doi.org/10.1117/12.824517
Event: SPIE Photonic Devices + Applications, 2009, San Diego, California, United States

Abstract

We discuss the use of genetically modified fluorescent proteins (FPs) and a specially engineered chromophore for nonlinear optical imaging. While it is clear that FPs can be used for two-photon fluorescence (TPF) microscopy, we show that they also exhibit a large second-order nonlinear optical response, useful for second-harmonic generation (SHG) imaging. The relation between the linear and nonlinear optical properties in a small series of FPs will be discussed. We also present a new optimized chromophore for combined TPF and SHG microscopy and we show imaging results obtained on this chromophore on a water droplet model system.

Citation Download Citation

Koen Clays, Jos Vanderleyden, and Harry L Anderson "Nano-engineering of materials for nonlinear optical imaging", Proc. SPIE 7413, Linear and Nonlinear Optics of Organic Materials IX, 74130J (20 August 2009); https://doi.org/10.1117/12.824517

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE