Photoacoustic tomography of pathological tissue in ex vivo mouse hearts

Markus Holotta; Harald Grossauer; Christian Kremser; Pavle Torbica; Jakob Völkl; Gerald Degenhart; Regina Esterhammer M.D.; Robert Nuster; Günther Paltauf; Werner Jaschke M.D.

doi:10.1117/12.842032

23 February 2010 Photoacoustic tomography of pathological tissue in ex vivo mouse hearts

Markus Holotta, Harald Grossauer, Christian Kremser, Pavle Torbica, Jakob Völkl, Gerald Degenhart, Regina Esterhammer M.D., Robert Nuster, Günther Paltauf, Werner Jaschke M.D.

Author Affiliations +

Proceedings Volume 7564, Photons Plus Ultrasound: Imaging and Sensing 2010; 75642X (2010) https://doi.org/10.1117/12.842032
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

In the present study, we evaluate the applicability of ex-vivo photoacoustic imaging (PAI) in organs of small animals. We used photoacoustic tomography (PAT) to visualize infarcted areas within mouse hearts and compared it to other imaging techniques (MRI and μCT). In order to induce ischemia an in-vivo ligation of the Ramus interventricularis anterior (RIVA, left anterior descending, LAD) was performed on nine wild type C41 mice. After varying survival periods the mice were sacrificed. The hearts were excised and immediately transferred into a formaldehyde solution for conservation. Various wavelengths in the visible and near infrared region (500 nm - 1000 nm) had been tested to find the best representation of the ischemic regions. Samples were illuminated with nanosecond laser pulses delivered by an Nd:YAG pumped optical parametric oscillator. Ultrasound detection was achieved by an optical Mach-Zehnder interferometer working as an integrating line detector. For acoustic coupling the samples were located inside a water tank. The voxel data are computed from the measurement data by a Fourier-domain based reconstruction algorithm, followed by a sequence of inverse Radon transforms. Results clearly show the capability of PAI to detect pathological tissue and the possibility to produce three-dimensional images with resolutions well below 100 μm. Different wavelengths allow the representation of structure inside an organ or on the surface even without contrast enhancing tracers.

Citation Download Citation

Markus Holotta, Harald Grossauer, Christian Kremser, Pavle Torbica, Jakob Völkl, Gerald Degenhart, Regina Esterhammer M.D., Robert Nuster, Günther Paltauf, and Werner Jaschke M.D. "Photoacoustic tomography of pathological tissue in ex vivo mouse hearts", Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75642X (23 February 2010); https://doi.org/10.1117/12.842032

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Heart

Tissues

Magnetic resonance imaging

Tissue optics

Optical testing

Photoacoustic tomography

Acquisition tracking and pointing

Show All Keywords

Keywords/Phrases

Search In:

Publication Years