In vivo functional photoacoustic micro-imaging of the electrically stimulated rat brain with multiwavelengths

Lun-De Liao; Meng-Lin Li; Hsin-Yi Lai; You-Yin Chen; Paul C.-P. Chao; Po-Hsun Wang

doi:10.1117/12.843085

23 February 2010 In vivo functional photoacoustic micro-imaging of the electrically stimulated rat brain with multiwavelengths

Lun-De Liao, Meng-Lin Li, Hsin-Yi Lai, You-Yin Chen, Paul C.-P. Chao, Po-Hsun Wang

Author Affiliations +

Proceedings Volume 7564, Photons Plus Ultrasound: Imaging and Sensing 2010; 75643F (2010) https://doi.org/10.1117/12.843085
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

In this study, we report on using multi-wavelength photoacoustic microscopy to image hemodynamic changes of total hemoglobin concentration (HbT) (i.e., blood volume) and oxygenation (SO2) in rat brain cortex vessels with electrical stimulation. Electrical stimulation of the rat left forelimb was applied to evoke changes in vascular dynamics of the rat somatosensory cortex. The applied current pulses were with a pulse frequency of 3 Hz, pulse duration of 0.2 ms, and pulse amplitude of 5 mA, respectively. The imaging target of rat brains was demarcated at AP 0 - -2.5 mm and ML ± 6 mm with respect to bregma. HbT changes were probed by images acquired at 570 nm, a hemoglobin isosbestic point while SO₂ changes were imaged by those acquired at 560 nm or 600 nm and their derivatives, which were normalized to those with 570 nm wavelengths. Correlation between the electrical stimulation paradigm and images acquired at 570, 560, and 600 nm in contralateral and ipsilateral vasculature was statistically analyzed, showing that the HbT and SO₂ changes revealed by multi-wavelength photoacoustic images spatially correlated with contralateral vasculature.

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Lun-De Liao, Meng-Lin Li, Hsin-Yi Lai, You-Yin Chen, Paul C.-P. Chao, and Po-Hsun Wang "In vivo functional photoacoustic micro-imaging of the electrically stimulated rat brain with multiwavelengths", Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75643F (23 February 2010); https://doi.org/10.1117/12.843085

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