Light scattering measurements for quantifying biological cell concentration: an optimization of opto-geometric parameters

A. G. Mignani; L. Ciaccheri; A. A. Mencaglia; L. Giannelli

doi:10.1117/12.884814

17 May 2011 Light scattering measurements for quantifying biological cell concentration: an optimization of opto-geometric parameters

A. G. Mignani, L. Ciaccheri, A. A. Mencaglia, L. Giannelli

Author Affiliations +

Proceedings Volume 7753, 21st International Conference on Optical Fiber Sensors; 77536A (2011) https://doi.org/10.1117/12.884814
Event: 21st International Conference on Optical Fibre Sensors (OFS21), 2011, Ottawa, Canada

Abstract

An experimental study was carried out, aimed at optimizing the optical/geometrical configuration for measuring the concentration of biological cells by means of static light scattering measurements. A LED-based optoelectronic setup making use of optical fibers was experimented, as the precursor of a low-cost device to be integrated in instrumentation for cytometry. Two biological sample types were considered as test samples of the most popular analyses - cervical cells and urine, respectively. The most suitable wavelengths and detecting angles were identified, and calibration curves were calculated.

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A. G. Mignani, L. Ciaccheri, A. A. Mencaglia, and L. Giannelli "Light scattering measurements for quantifying biological cell concentration: an optimization of opto-geometric parameters", Proc. SPIE 7753, 21st International Conference on Optical Fiber Sensors, 77536A (17 May 2011); https://doi.org/10.1117/12.884814

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