Spectral characteristics of two-photon autofluorescence and second harmonic generation from human skin in vivo

Hans Georg Breunig; Karsten König

doi:10.1117/12.874990

17 February 2011 Spectral characteristics of two-photon autofluorescence and second harmonic generation from human skin in vivo

Hans Georg Breunig, Karsten König

Author Affiliations +

Proceedings Volume 7883, Photonic Therapeutics and Diagnostics VII; 788311 (2011) https://doi.org/10.1117/12.874990
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

We performed multiphoton imaging of human skin and recorded in combination the complete spectral content of the signals in vivo. The spectra represent the integration of multiphoton signals over the investigated regions of the epidermis and dermis. They are used to study depth-resolved in vivo emission characteristics of main endogenous skin fluorophores like keratin, NAD(P)H, collagen and elastin. The identification of the specific fluorophores is supported by analysis of additional in vivo fluorescence lifetime imaging. Furthermore, as a potential application of spectrally selective imaging the possibility to investigate the penetration of nanoparticles from sunscreen lotion into skin in vivo is discussed.

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Hans Georg Breunig and Karsten König "Spectral characteristics of two-photon autofluorescence and second harmonic generation from human skin in vivo", Proc. SPIE 7883, Photonic Therapeutics and Diagnostics VII, 788311 (17 February 2011); https://doi.org/10.1117/12.874990

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