Combining multiphoton and CARS microscopy for skin imaging

H. G. Breunig; M. Weinigel; J. Lademann; W. Sterry; I. Latka; B. Dietzek; J. Popp; K. König

doi:10.1117/12.874969

11 February 2011 Combining multiphoton and CARS microscopy for skin imaging

H. G. Breunig, M. Weinigel, J. Lademann, W. Sterry, I. Latka, B. Dietzek, J. Popp, K. König

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 79031A (2011) https://doi.org/10.1117/12.874969
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

We present combined coherent anti Stokes Raman scattering and two-photon excited fluorescence microscopy, for label-free in vitro and in vivo imaging of skin with both chemical discrimination and subcellular resolution. In particular, we show how CARS microscopy is utilized to image lipid-rich structures inside the skin, preparing for a combined multiphoton and CARS imaging modality for biomedical research and skin imaging. As a further potential application the detection of topically applied oil on the skin is presented.

Citation Download Citation

H. G. Breunig, M. Weinigel, J. Lademann, W. Sterry, I. Latka, B. Dietzek, J. Popp, and K. König "Combining multiphoton and CARS microscopy for skin imaging", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031A (11 February 2011); https://doi.org/10.1117/12.874969

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