Frequency domain diffuse fluorescence tomography for detection of deep lesions

Uwe J. Netz; Ingo Gersonde; Jan Toelsner; Gerd Illing

doi:10.1117/12.889694

16 June 2011 Frequency domain diffuse fluorescence tomography for detection of deep lesions

Uwe J. Netz, Ingo Gersonde, Jan Toelsner, Gerd Illing

Author Affiliations +

Proceedings Volume 8088, Diffuse Optical Imaging III; 80881O (2011) https://doi.org/10.1117/12.889694
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

In this paper we present two-dimensional phantom measurements of fluorescence light distribution in the frequency domain and reconstruction of three-dimensional fluorophore distribution. An experimental set-up was built up with two dimensional laser scanning, intensity modulation with frequencies up to 1 GHz, and two-dimensional imaging of modulated fluorescence light. Stable phantoms were developed simulating mammary tissue to perform measurements in a backscattering geometry for a variety of cylindrical fluorescence sources with different diameters, fluorophore concentrations, and surface distances at different modulation frequencies. At first calculated fluorescence light distributions from Monte-Carlo simulations was compared to measured data. In a second step from tomographic data sets of calculated fluorescent light, three-dimensional tomographic reconstructions of fluorophore distribution were performed. Finally three-dimensional tomographic reconstructions of fluorophore distribution were performed from tomographic fluorescence measurements. We found good concurrence between measured and calculated fluorescence distribution. Synthetic and real tomographic reconstruction showed good localization but underestimated the depth of fluorophore distribution.

Citation Download Citation

Uwe J. Netz, Ingo Gersonde, Jan Toelsner, and Gerd Illing "Frequency domain diffuse fluorescence tomography for detection of deep lesions", Proc. SPIE 8088, Diffuse Optical Imaging III, 80881O (16 June 2011); https://doi.org/10.1117/12.889694

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