A fluorescence polarization based assay for glucose sensing

Brian M. Cummins; Gerard L. Coté

doi:10.1117/12.908630

14 February 2012 A fluorescence polarization based assay for glucose sensing

Brian M. Cummins, Gerard L. Coté

Proceedings Volume 8229, Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV; 82291C (2012) https://doi.org/10.1117/12.908630
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

A fluorescence polarization (FP) assay was developed to determine concentrations of glucose using concanavalin A (ConA) and fluorescently-labeled dextran. Predictive FP responses to glucose were elicited for different assay configurations using mathematical modeling and displayed herein. Using 4 kDa FITC-dextran, we predicted a change of 0.120 P units from 0 mg/dL glucose to 500 mg/dL. This shows the potential that a homogenous, reproducible FP assay can be engineered to measure glucose concentrations using tetrameric ConA and 4k kDa FITC-dextran.

Citation Download Citation

Brian M. Cummins and Gerard L. Coté "A fluorescence polarization based assay for glucose sensing", Proc. SPIE 8229, Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV, 82291C (14 February 2012); https://doi.org/10.1117/12.908630

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