Photon absorption in step-wise multi-photon activation fluorescence (SMPAF) of Sepia melanin

Zhenhua Lai; Josef Kerimo; Charles DiMarzio

doi:10.1117/12.2001384

22 February 2013 Photon absorption in step-wise multi-photon activation fluorescence (SMPAF) of Sepia melanin

Zhenhua Lai, Josef Kerimo, Charles DiMarzio

Author Affiliations +

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 85881I (2013) https://doi.org/10.1117/12.2001384
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Previous research has shown that melanin goes through a step-wise three-photon absorption process when the fluorescence is activated with high laser intensity. We have conducted further research using even higher laser intensity for the activation, and have shown the possibility of observing power dependence other than third-order. This article discusses the possible energy states of Sepia melanin by studying the power dependence curves of the step-wise multi-photon activated fluorescence signal. Three different excitation channels are activated. Possible reasons causing the three channels are discussed.

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Zhenhua Lai, Josef Kerimo, and Charles DiMarzio "Photon absorption in step-wise multi-photon activation fluorescence (SMPAF) of Sepia melanin ", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85881I (22 February 2013); https://doi.org/10.1117/12.2001384

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