Light sheet adaptive optics microscope for 3D live imaging

C. Bourgenot; J. M. Taylor; C. D. Saunter; J. M. Girkin; G. D. Love

doi:10.1117/12.2002057

22 February 2013 Light sheet adaptive optics microscope for 3D live imaging

C. Bourgenot, J. M. Taylor, C. D. Saunter, J. M. Girkin, G. D. Love

Proceedings Volume 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX; 85890W (2013) https://doi.org/10.1117/12.2002057
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

We report on the incorporation of adaptive optics (AO) into the imaging arm of a selective plane illumination microscope (SPIM). SPIM has recently emerged as an important tool for life science research due to its ability to deliver high-speed, optically sectioned, time-lapse microscope images from deep within in vivo selected samples. SPIM provides a very interesting system for the incorporation of AO as the illumination and imaging paths are decoupled and AO may be useful in both paths. In this paper, we will report the use of AO applied to the imaging path of a SPIM, demonstrating significant improvement in image quality of a live GFP-labeled transgenic zebrafish embryo heart using a modal, wavefront sensorless approach and a heart synchronization method. These experimental results are linked to a computational model showing that significant aberrations are produced by the tube holding the sample in addition to the aberration from the biological sample itself.

Citation Download Citation

C. Bourgenot, J. M. Taylor, C. D. Saunter, J. M. Girkin, and G. D. Love "Light sheet adaptive optics microscope for 3D live imaging", Proc. SPIE 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, 85890W (22 February 2013); https://doi.org/10.1117/12.2002057

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