Optical nanoscopy of a living cell

Balpreet Singh Ahluwalia; Deanna L. Wolfson; Frank Y. S. Chuang; Thomas Huser

doi:10.1117/12.2062581

19 November 2014 Optical nanoscopy of a living cell

Balpreet Singh Ahluwalia, Deanna L. Wolfson, Frank Y. S. Chuang, Thomas Huser

Proceedings Volume 9169, Nanoimaging and Nanospectroscopy II; 91690V (2014) https://doi.org/10.1117/12.2062581
Event: SPIE NanoScience + Engineering, 2014, San Diego, California, United States

Abstract

Optical nanoscopy allows to study biological and functional processes of sub-cellular organelles. In structured illumination microscopy (SIM) the sample is illuminated with a grid-like interference pattern to encode higher spatial frequency information into observable Moiré patterns. By acquiring multiple images and a computation trick a superresolved image is obtained. SIM provides resolution enhancement of 2X in each axis as compared to conventional microscopes. For a visible light, SIM provides an optical resolution of 100 nm. The challenges associated with optical nanoscopy of a living cell are photo-toxicity, special dye requirements and artifacts due to cell movement. SIM works with conventional dyes and is a wide-field technique making it suitable for imaging living cells. In this work, we will discuss the opportunities and challenges of imaging living cells using SIM. Two applications of optical nanoscopy of living cells will be discussed; a) imaging of mitochondria in a keratinocyte cell and Optical microscopy based on fluorescence has emerged as a vital tool in modern bio-medical imaging and diagnosis. Super-resolution bio-imaging allows gathering information from sub-cellular organelles. In structured illumination microscopy (SIM) the sample is illuminated with a grid-like interference patterns to encode higher spatial frequencies information into observable images (Moiré fringes). A super-resolved image is then decoded using computational trick. In this work, we used SIM to acquired super-resolved optical images of mitochondria from a live keratinocyte cell (see Fig 1). SIM provides resolution enhancement of 2X in each axis and contrast enhancement of 8X on a projected image. Time-lapsed imaging was used to study the dynamics of mitochondria in a live cell.

Citation Download Citation

Balpreet Singh Ahluwalia, Deanna L. Wolfson, Frank Y. S. Chuang, and Thomas Huser "Optical nanoscopy of a living cell", Proc. SPIE 9169, Nanoimaging and Nanospectroscopy II, 91690V (19 November 2014); https://doi.org/10.1117/12.2062581

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
4 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Microscopy

Optical resolution

Super resolution microscopy

Optical transfer functions

Resolution enhancement technologies

Stimulated emission depletion microscopy

Luminescence

Show All Keywords

Keywords/Phrases

Search In:

Publication Years