Determination of the spectral dependence of reduced scattering and quantitative second-harmonic generation imaging for detection of fibrillary changes in ovarian cancer

Kirby R. Campbell; Karissa B. Tilbury; Paul J. Campagnola

doi:10.1117/12.2083962

5 March 2015 Determination of the spectral dependence of reduced scattering and quantitative second-harmonic generation imaging for detection of fibrillary changes in ovarian cancer

Kirby R. Campbell, Karissa B. Tilbury, Paul J. Campagnola

Author Affiliations +

Proceedings Volume 9329, Multiphoton Microscopy in the Biomedical Sciences XV; 93292A (2015) https://doi.org/10.1117/12.2083962
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Here, we examine ovarian cancer extracellular matrix (ECM) modification by measuring the wavelength dependence of optical scattering measurements and quantitative second-harmonic generation (SHG) imaging metrics in the range of 800-1100 nm in order to determine fibrillary changes in ex vivo normal ovary, type I, and type II ovarian cancer. Mass fractals of the collagen fiber structure is analyzed based on a power law correlation function using spectral dependence measurements of the reduced scattering coefficient μ_s′ where the mass fractal dimension is related to the power. Values of μ_s′ are measured using independent methods of determining the values of μ_sand g by on-axis attenuation measurements using the Beer-Lambert Law and by fitting the angular distribution of scattering to the Henyey-Greenstein phase function, respectively. Quantitativespectral SHG imaging on the same tissues determines F_SHG/B_SHG creation ratios related to size and harmonophore distributions. Both techniques probe fibril packing order, but the optical scattering probes structures of sizes from about 50-2000 nm where SHG imaging – although only able to resolve individual fibers – builds contrast from the assembly of fibrils. Our findings suggest that type I ovarian tumor structure has the most ordered collagen fibers followed by normal ovary then type II tumors showing the least order.

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Kirby R. Campbell, Karissa B. Tilbury, and Paul J. Campagnola "Determination of the spectral dependence of reduced scattering and quantitative second-harmonic generation imaging for detection of fibrillary changes in ovarian cancer", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93292A (5 March 2015); https://doi.org/10.1117/12.2083962

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KEYWORDS

Second-harmonic generation

Scattering

Tissues

Ovarian cancer

Collagen

Tissue optics

Tumors

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