Hardware-friendly bi-exponential fluorescence lifetime imaging algorithms and phasor approaches

David Li; Yu Chen

doi:10.1117/12.2183082

14 July 2015 Hardware-friendly bi-exponential fluorescence lifetime imaging algorithms and phasor approaches

David Li, Yu Chen

Proceedings Volume 9536, Advanced Microscopy Techniques IV; and Neurophotonics II; 95360M (2015) https://doi.org/10.1117/12.2183082
Event: European Conferences on Biomedical Optics, 2015, Munich, Germany

Abstract

A newly developed hardware-friendly non-iterative fluorescence lifetime imaging (FLIM) analysis method was verified in an FPGA chip. Its performances were also demonstrated on two-photon FLIM images of gold nanorods (GNRs)-Cy5 labelled Hela cells. The results obtained by the proposed method can be presented in a polor plot to be compared to the widely used phasor (Phasor) approach. Combining our method with Phasor will be very useful in FLIM analysis.

Citation Download Citation

David Li and Yu Chen "Hardware-friendly bi-exponential fluorescence lifetime imaging algorithms and phasor approaches", Proc. SPIE 9536, Advanced Microscopy Techniques IV; and Neurophotonics II, 95360M (14 July 2015); https://doi.org/10.1117/12.2183082

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